小鼠血管生成素1(ANGPT1)測定試劑盒
中文名稱:小鼠血管生成素1(ANGPT1)測定試劑盒
本試劑盒應用雙抗體夾心法測定標本中小鼠血管生成素1(ANGPT1)測定試劑盒水平。用純化的抗體包被微孔板,制成固相抗體,往包被單抗的微孔中依次加入,再與HRP標記的結合,形成抗體-抗原-酶標抗體復合物,經過*洗滌后加底物TMB顯色。TMB在HRP酶的催化下轉化成藍色,并在酸的作用下轉化成zui終的黃色。顏色的深淺和樣品中的呈正相關。用酶標儀在450nm波長下測定吸光度(OD值),通過標準曲線計算樣品中的濃度。
The E-Z 96? Mag-Bind? Tissue RNA Kit provides a novel technology for total RNA isolation from plant and animal tissues. This kit allows the rapid and reliable isolation of high-quality total cellular RNA from a wide variety of tissues. Samples are first lysed in a specially formulated buffer and then proteins and DNA are precipitated. The Supertanent is combined with magentic beads and after a couple of quick wash steps pure RNA is eluted. Unlike column-based systems, the binding of nucleic acids to magnetic partciles occurs in solution, resulting in increased binding kinetics and binding efficiency. Particles are also completly re-suspended during the wash steps of the purification protocol, which enhances contact with the wash buffer and removal of contaminants and increasing nucleic acid purity. E.Z.N.A.? Mag-Bind? Tissue RNA procedure can be automated with most robotic workstations。
北京方程生物公司是一家專業經營生物、儀器、試劑、耗材的公司,在廣大用戶的幫助和支持下,經過不懈的努力,已成為國內生命科學領域產品的主要供應商之一,代理許多*水平的高科技產品,包括分子生物學、細胞生物學、免疫學、診斷等多個研究及應用領域.公司的服務和業務網絡遍及全國,在同行獲得*好評. 特別是生物試劑和細胞產品更是種類齊全、價格實惠,*原代細胞,專業培養基,常用傳統培養基,細胞培養用的相關試劑,氨基酸,抗生素,維他命類產品品種齊全,試劑耗材種類繁多.。
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