大鼠尼克酰胺腺嘌呤二核苷酸磷酸氧化酶4(NOX4)測(cè)定試劑盒
中文名稱(chēng):大鼠尼克酰胺腺嘌呤二核苷酸磷酸氧化酶4(NOX4)測(cè)定試劑盒
本試劑盒應(yīng)用雙抗體夾心法測(cè)定標(biāo)本中大鼠尼克酰胺腺嘌呤二核苷酸磷酸氧化酶4(NOX4)測(cè)定試劑盒水平。用純化的抗體包被微孔板,制成固相抗體,往包被單抗的微孔中依次加入,再與HRP標(biāo)記的結(jié)合,形成抗體-抗原-酶標(biāo)抗體復(fù)合物,經(jīng)過(guò)*洗滌后加底物TMB顯色。TMB在HRP酶的催化下轉(zhuǎn)化成藍(lán)色,并在酸的作用下轉(zhuǎn)化成zui終的黃色。顏色的深淺和樣品中的呈正相關(guān)。用酶標(biāo)儀在450nm波長(zhǎng)下測(cè)定吸光度(OD值),通過(guò)標(biāo)準(zhǔn)曲線計(jì)算樣品中的濃度。
The E-Z 96? Mag-Bind? Tissue RNA Kit provides a novel technology for total RNA isolation from plant and animal tissues. This kit allows the rapid and reliable isolation of high-quality total cellular RNA from a wide variety of tissues. Samples are first lysed in a specially formulated buffer and then proteins and DNA are precipitated. The Supertanent is combined with magentic beads and after a couple of quick wash steps pure RNA is eluted. Unlike column-based systems, the binding of nucleic acids to magnetic partciles occurs in solution, resulting in increased binding kinetics and binding efficiency. Particles are also completly re-suspended during the wash steps of the purification protocol, which enhances contact with the wash buffer and removal of contaminants and increasing nucleic acid purity. E.Z.N.A.? Mag-Bind? Tissue RNA procedure can be automated with most robotic workstations。
產(chǎn)品說(shuō)明:方便-處理簡(jiǎn)單, 樣品不再需要超低溫貯藏靈活-純化RNA時(shí),只需要將樣品取出即可可靠-該保護(hù)液是一種新型的溶液,能快速滲透到細(xì)胞或組織內(nèi)部,高效地抑制內(nèi)源性RNase酶的活性。
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