試劑盒名稱:小鼠淋巴細(xì)胞功能相關(guān)抗原3 LFA-3/CD58
Pig Glutamate Receptor, Ionotropic, AMPA 1 (GRIA1) ELISA
規(guī)格: 96T/48T
品牌:BIOFINE
種屬:人ELISA試劑盒
檢測(cè)波長(zhǎng):450nm
所需樣本體積: 50-100ul
適用范圍:僅供科研
保存及有效期:2-8℃，六個(gè)月，-20℃一年
檢測(cè)目的:用于測(cè)定血清，血漿及相關(guān)液體小鼠淋巴細(xì)胞功能相關(guān)抗原3 LFA-3/CD58含量。適合檢測(cè)包括血清、血漿、尿液、胸腹水、灌洗液、腦脊液、細(xì)胞培養(yǎng)上清、組織勻漿等標(biāo)本。
ELISA results using S-OIV A neuraminidase antibody at 1 μg/ml to probe the immunogenic and the corresponding seasonal influenza A neuraminidase peptides at 50, 10, 2, and 0 ng/ml. Because the ELISA can be performed to evaluate either the presence of antigen or the presence of antibody in a sample, it is a useful tool for determining serum antibody concentrations (such as with the HIV test[3] or West Nile Virus). It has also found applications in the food industry in detecting potential food allergens such as milk, peanuts, walnuts, almonds, and eggs.[4] ELISA can also be used in toxicology as a rapid presumptive screen for certain classes of drugs. The ELISA was the first screening test widely used for HIV because of its high sensitivity. In an ELISA, a person's serum is diluted 400-fold and applied to a plate to which HIV antigens are attached. If antibodies to HIV are present in the serum, they may bind to these HIV antigens. The plate is then washed to remove all other components of the serum. A specially prepared "secondary antibody" — an antibody that binds to other antibodies — is then applied to the plate, followed by another wash. This secondary antibody is chemically linked in advance to an enzyme. Thus, the plate will contain enzyme in proportion to the amount of secondary antibody bound to the plate. A substrate for the enzyme is applied, and catalysis by the enzyme leads to a change in color or fluorescence. ELISA results are reported as a number; the most controversial aspect of this test is determining the "cut-off" point between a positive and a negative result. A cut-off point may be determined by comparing it with a known standard. If an ELISA test is used for drug screening at workplace, a cut-off concentration, 50 ng/mL, for example, is established, and a sample that contains the standard concentration of analyte will be prepared. Unknowns that generate a signal that is stronger than the known sample are "positive." Those that generate weaker signal are "negative." Doctor Dennis E Bidwell and Alister Voller created the test.
結(jié)果判定：試驗(yàn)有效性：陽(yáng)性對(duì)照孔平均值≥1.00; 陰性對(duì)照平均值≤0.10臨界值（CUT OFF）計(jì)算：臨界值=陰性對(duì)照孔平均值+0.15陰性判定： 樣品 OD 值< 臨界值 （CUT OFF） 者為人 EB 病毒 Rta 蛋白抗體 IgG(Rta-IgG)陰性陽(yáng)性判定： 樣品 OD 值≥ 臨界值 （CUT OFF） 者為人 EB 病毒 Rta 蛋白抗體 IgG(Rta-IgG)陽(yáng)性注意事項(xiàng)1．操作嚴(yán)格按照說(shuō)明書進(jìn)行，本試劑不同批號(hào)組分不得混用。2．試劑盒從冷藏環(huán)境中取出應(yīng)在室溫平衡 15-30 分鐘后方可使用，酶標(biāo)包被板開封后如未用完，板條應(yīng)裝入密封袋中保存。3．濃洗滌液可能會(huì)有結(jié)晶析出，稀釋時(shí)可在水浴中加溫助溶，洗滌時(shí)不影響結(jié)果。4． 封板膜只限一次性使用，以避免交叉污染。
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