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北京方程嘉鴻科技有限公司
北京方程嘉鴻科技有限公司
北京方程生物公司經營銷售T細胞受體（TCR）ELISA試劑盒，可以提供免費代測，送檢贈京東卡活動正在進行中，詳情咨詢銷售人員
試劑盒名稱:T細胞受體(TCR)
IPO-38蛋白
規格: 96T/48T
品牌:BIOFINE
種屬:人ELISA試劑盒
檢測波長:450nm
所需樣本體積: 50-100ul
適用范圍:僅供科研
保存及有效期:2-8℃，六個月，-20℃一年
檢測目的:用于測定血清，血漿及相關液體T細胞受體(TCR)含量。適合檢測包括血清、血漿、尿液、胸腹水、灌洗液、腦脊液、細胞培養上清、組織勻漿等標本。
Enzyme-linked immunosorbent assay (ELISA), also known as an enzyme immunoassay (EIA), is a biochemical technique used mainly in immunology to detect the presence of an antibody or an antigen in a sample. The ELISA has been used as a diagnostic tool in medicine and plant pathology, as well as a quality-control check in various industries. In simple terms, in ELISA, an unknown amount of antigen is affixed to a surface, and then a specific antibody is applied over the surface so that it can bind to the antigen. This antibody is linked to an enzyme, and in the final step a substance is added that the enzyme can convert to some detectable signal, most commonly a colour change in a chemical substrate. Performing an ELISA involves at least one antibody with specificity for a particular antigen. The sample with an unknown amount of antigen is immobilized on a solid support (usually a polystyrene microtiter plate) either non-specifically (via adsorption to the surface) or specifically (via capture by another antibody specific to the same antigen, in a "sandwich" ELISA). After the antigen is immobilized, the detection antibody is added, forming a complex with the antigen. The detection antibody can be covalently linked to an enzyme, or can itself be detected by a secondary antibody that is linked to an enzyme through bioconjugation. Between each step, the plate is typically washed with a mild detergent solution to remove any proteins or antibodies that are not specifically bound. After the final wash step, the plate is developed by adding an enzymatic substrate to produce a visible signal, which indicates the quantity of antigen in the sample. Traditional ELISA typically involves chromogenic reporters and substrates that produce some kind of observable color change to indicate the presence of antigen or analyte. Newer ELISA-like techniques utilize fluorogenic, electrochemiluminescent, and real-time PCR reporters to create quantifiable signals. These new reporters can have various advantages including higher sensitivities and multiplexing.[1][2] In technical terms, newer assays of this type are not strictly ELISAs, as they are not "enzyme-linked" but are instead linked to some non-enzymatic reporter. However, given that the general principles in these assays are largely similar, they are often grouped in the same category as ELISAs.
T細胞受體(TCR)elisa試劑盒回收率是反應待測物在樣品分析過程中的損失的程度，損失越少，回收率越高，如果作標液1PPM，就是1毫克/升，而作出標準數據為0.99毫克/升，就是說你的回收率是99%，這個與真實成分有密切的關系，說明方法的準確度。比如水中總無機氯含量測定，樣品水中含有無機氯20mg/L，取100mL被測水樣品，加入0.1mL濃度為10mg/mL的含無機氯標準樣品，測定時忽略體積變化，如果測定出樣品中無機氯為2.98mg/L，則認為回收率為99%。
T細胞受體(TCR)
抗著絲點抗體(ACA/CENP)
CD4分子(CD4)
免疫抑制酸性蛋白(IAP)
低密度脂蛋白(LDL)
抗組蛋白(H2A-H2B)-DNA抗體/抗二聚體-DNA抗體
P物質受體(SP-R)
腮腺炎病毒IgG
游離β絨毛膜*(f-βhCG)
抗Q熱抗體(anti-Q-Ab)
乙型肝炎病毒X蛋白相互作用蛋白(HBXIP)
巨噬細胞炎性蛋白3α(MIP-3α/CCL20)
胰島素自身抗體(IAA)
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