Cyclic AMP (cAMP) is one of the most important “second messengers" involved as a modulator of physiological processes. A number of hormones are known to activate cAMP through the action of the enzyme Adenylate cyclase which converts ATP to cAMP. cAMP has been shown to be involved in the cardiovascular and nervous systems, immune mechanisms, cell growth and differentiation, and general metabolism.

Direct cAMP ELISA kit：

Most sensitive (0.006 pmol/ml) colorimetric kit for intracellular cAMP quantification.

Replaces Prod. #: ADI-900-066

Alternative size available: ADI-901-066A (5x96 wells)

Highly sensitive with an optional acetylation protocol which increases sensitivity >10-fold

High throughput format, results for up to 39 samples in duplicate in just 3 hours

Flexible assay, species independent

Easy-to-use pre-coated plate and liquid color-coded reagents reduce errors

Efficient and well-established sample handling protocols

Widely cited in peer-reviewed publications

Product Details：

Alternative Name: cyclic AMP

Sensitivity: Non-acetylated: 1.18 pmol/ml (range 0.78 - 200 pmol/ml)

Acetylated: 0.006 pmol/ml (range 0.078 - 20 pmol/ml)

Assay Time: 3 hours

Applications: ELISA, Colorimetric detection

Application Notes: For the quantitative determination of cAMP in cell lysates and tissue from any species. Cited sample types include tissue culture.

Wavelength: 405 nm

Species reactivity: Species independent

Crossreactivity: cyclic AMP (100%)

AMP, ATP, cyclic GMP, GMP, GTP, cyclic UMP, CTP (<0.001%)

Use/Stability: Store all components at +4°C, except standard and conjugate at -20°C.

Shipping: Shipped on Blue Ice

Contents: GxR IgG Microtiter plate, Conjugate, Antibody, 0.1M HCl, Neutralizing reagent, Wash buffer concentrate, Standard, pNpp Substrate, Stop solution, Acetic anhydride, Triethylamine

ADI-900-066A cAMP直接ELISA試劑盒用于細(xì)胞內(nèi)cAMP定量的靈敏（0.006 pmol/ml）比色試劑盒。這是一種比色競爭免疫分析試劑盒，用于定量測定在0.1M HCl中溶解的細(xì)胞或組織中的細(xì)胞內(nèi)cAMP，該試劑盒用于停止內(nèi)源性磷酸二酯酶活性并穩(wěn)定釋放的環(huán)AMP。然后在微量滴定板中直接分析0.1M HCl處理過的樣品，無需提取、干燥和重構(gòu)。乙酰化（包括試劑）使靈敏度提高10倍以上。在405 nm處讀取吸光度。與競爭對(duì)手相比，大的信號(hào)背景比提供了*的靈敏度。該試劑盒可讓你通過GPCR輕松評(píng)估腺苷酸環(huán)化酶的活性。

艾美捷ADI-900-066A cAMP直接ELISA試劑盒基本參數(shù)：

備選名稱：循環(huán)AMP

靈敏度：非乙酰化：1.18 pmol/ml（范圍0.78-200 pmol/ml）

乙酰化：0.006 pmol/ml（范圍0.078-20 pmol/ml）

化驗(yàn)時(shí)間：3小時(shí)

應(yīng)用：ELISA，比色檢測

應(yīng)用說明：用于定量測定任何物種的細(xì)胞裂解物和組織中的cAMP。引用的樣本類型包括組織培養(yǎng)。

波長：405 nm

物種反應(yīng)性：物種無關(guān)

交叉反應(yīng)性：環(huán)狀AMP（100%）

AMP、ATP、環(huán)狀GMP、GMP、GTP、環(huán)狀UMP、CTP（<0.001%）

使用/穩(wěn)定性：在+4°C下儲(chǔ)存所有成分，-20°C下標(biāo)準(zhǔn)和共軛物除外。

運(yùn)輸：藍(lán)冰運(yùn)輸

內(nèi)容：GxR IgG微量滴定板、綴合物、抗體、0.1M HCl、中和試劑、洗滌緩沖液濃縮液、標(biāo)準(zhǔn)品、pNpp底物、停止溶液、乙酸酐、三乙胺

cAMP直接ELISA試劑盒文獻(xiàn)參考：

1.骨橋蛋白缺失增強(qiáng)心肌細(xì)胞β2-腎上腺素能受體依賴性抗纖維化信號(hào)傳導(dǎo)：C.M.Pollard等。國際分子科學(xué)雜志。20, 1396 (2019);

2.培養(yǎng)基中葡萄糖濃度升高通過cAMP/PKA依賴性途徑減少LLC-PK1細(xì)胞中SGLT2的膜轉(zhuǎn)運(yùn)：S.Sunilkumar等人。美國生理學(xué)雜志。細(xì)胞生理學(xué)。316，C913（2019）；

3.在成年小鼠的實(shí)驗(yàn)?zāi)Ｐ椭校?氨-酸-甘-氨-酸和肌肽二肽可預(yù)防由多次低劑量鏈脲佐菌素誘導(dǎo)的糖尿病：T.Vahdatpour等人。J、 糖尿病研究。10，1177（2019），應(yīng)用：雄性BALB/c小鼠血液樣本中的cAMP水平