The 9H10 monoclonal antibody reacts with mouse CTLA-4 (cytotoxic T lymphocyte antigen-4) also known as CD152. CTLA-4 is a 33 kDa cell surface receptor encoded by the Ctla4 gene that belongs to the CD28 family of the Ig superfamily. CTLA-4 is expressed on activated T and B lymphocytes. CTLA-4 is structurally similar to the T-cell co-stimulatory protein, CD28, and both molecules bind to the B7 family members B7-1 (CD80) and B7-2 (CD86). Upon ligand binding, CTLA-4 negatively regulates cell-mediated immune responses. CTLA-4 plays roles in induction and/or maintenance of immunological tolerance, thymocyte development, and regulation of protective immunity. The critical role of CTLA-4 in immune down-regulation has been demonstrated in CTLA-4 deficient mice, which succumb at 3-5 weeks of age due to the development of a lymphoproliferative disease. CTLA-4 is among a group of inhibitory receptors being explored as cancer treatment targets through immune checkpoint blockade. The 9H10 antibody has been shown to promote T cell co-stimulation by blocking CTLA-4 binding to the B7 co-receptors, allowing for CD28 binding.

9H10單克隆抗體與小鼠CTLA-4（細胞毒性T淋巴細胞抗原-4）反應，也稱為CD152。CTLA-4是由Ctla33基因編碼的4 kDa細胞表面受體，屬于Ig超家族的CD28家族。CTLA-4在活化的T和B淋巴細胞上表達。CTLA-4在結構上與T細胞共刺激蛋白CD28相似，并且兩種分子都與B7家族成員B7-1（CD80）和B7-2（CD86）結合。配體結合后，CTLA-4負調節細胞介導的免疫反應。CTLA-4在誘導和/或維持免疫耐受、胸腺細胞發育和保護性免疫調節中發揮作用。CTLA-4在免疫下調中的關鍵作用已在CTLA-4缺陷小鼠中得到證實，由于淋巴組織增生性疾病的發展，這些小鼠在3-5周齡時死亡。CTLA-4是一組通過免疫檢查點阻斷作為癌癥治療靶點的抑制受體之一。9H10抗體已被證明通過阻斷CTLA-4與B7共受體的結合來促進T細胞共刺激，從而允許CD28結合。

艾美捷InVivoPlus抗小鼠CTLA-4 (CD152) #BP0131、#BP0032規格：

同種型 敘利亞倉鼠IgG

推薦的同種型對照 體內加多克隆敘利亞倉鼠 IgG

推薦的稀釋緩沖液 體內純 pH 7.0 稀釋緩沖液

免疫原 小鼠CTLA-4-人IgG1融合蛋白

報告的應用程序 體內CTLA-4 體外中和 CTLA-4 中和

蛋白質印跡

配方 PBS，pH 7.0

不含穩定劑或防腐劑

內毒素* <1EU/mg （<0.001EU/μg）

通過LAL凝膠凝血測定法測定

集合體* <5%

由 DLS 確定

純度 >95%

由 SDS-PAGE 決定

無 菌 0.2 μM 過濾

生產 從無動物設施中的組織培養上清液中純化

純化 蛋白 G

里德 AB_10950184

分子量 150 千達

小鼠病原體檢測* 外切粒菌/鼠痘病毒： 陰性 漢坦病毒： 陰性 K 病毒： 陰性乳酸脫氫酶升高病毒： 陰性 淋巴細胞性脈絡叢腦膜炎病毒： 陰性小鼠腺病毒： 陰性小鼠巨細胞病毒： 陰性小鼠肝炎病毒： 陰性小鼠分鐘病毒： 陰性小鼠諾如病毒： 陰性小鼠細小病毒： 陰性

小鼠輪狀病毒：肺支原體陰性：陰性 小鼠肺炎病毒：陰性 多瘤病毒：陰性呼腸孤病毒篩查：陰性 仙臺病毒：陰性 泰勒鼠腦脊髓炎：陰性

存儲 抗體溶液應以4°C的儲備濃度儲存。 不要凍結。

InVivoPlus抗小鼠CTLA-4 (CD152) 部分文獻引用：

in vivo CTLA-4 neutralization

Hervieu, A., et al. (2013). "Dacarbazine-mediated upregulation of NKG2D ligands on tumor cells activates NK and CD8 T cells and restrains melanoma growth" J Invest Dermatol 133(2): 499-508.

in vivo CTLA-4 neutralization

Goding, S. R., et al. (2013). "Restoring immune function of tumor-specific CD4+ T cells during recurrence of melanoma" J Immunol 190(9): 4899-4909.

in vivo CTLA-4 neutralization

Waitz, R., et al. (2012). "Potent induction of tumor immunity by combining tumor cryoablation with anti-CTLA-4 therapy" Cancer Res 72(2): 430-439.

in vivo CTLA-4 neutralization

Balachandran, V. P., et al. (2011). "Imatinib potentiates antitumor T cell responses in gastrointestinal stromal tumor through the inhibition of Ido" Nat Med 17(9): 1094-1100.