產(chǎn)品新聞
背景資料
Hypoxia-inducible factor-1 （HIF-1）， identified as one of the transcription factors， has been found to play an essential role in cellular and systemic oxygen homeostasis. HIF-1 is a heterodimer composed of HIF-1? subunit and one of three subunits （Hif-1?， Hif-2 （or Hif-3））. The activation of Hif-1 （is closely associated with a variety of tumors and oncogenic pathways. Hif-1 （consists of DNA binding domain （DBD domain）， Dimerization domain and C-terminal regulatory domains， including two transactivation domains （TAD）， an oxygen-dependent degradation （ODD） domain， and inhibitory domains. Under hypoxic conditions HIF1A activates the transcription of more than 40 genes， including， erythropoietin， glucose transporters， glycolytic enzymes， VEGF， and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF-1A also plays a crucial role in embryonic vascularization， tumor angiogenesis and pathophysiology of ischemic disease. It binds to core DNA sequence 5’-[AG]CGTG-3’ within the hypoxia response element （HRE） of target gene promoters. Activation involves recruitment of transcriptional coactivators such as CREBPB and EP300. Its activity is improved by interaction with both， NCOA1 or NCOA2. Interaction with redox regulatory protein APEX appears to activate CTAD and potentiates activation by NCOA1 and CREBBP. The induction is under reduced oxygen tension. HIF1A is also induced by a variety of receptor-mediated factors such as growth factors， cytokines， and circulatory factors for example PDGF， EGF， FGF-2， IGF-2， TGF-1 beta， HGF， TNF alpha， IL-1 beta， angiotensin-2 and thrombin. Nevertheless， this induction is less intense than that stimulated by hypoxia.
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來源宿主
大腸桿菌中重組表達(dá)
溶解建議
Sterile filtered liquid formulation. The HIF1A recombinant Human solution is formulated 20mM Tris-HCl pH-7.5 and 1mM DTT.
保存建議
MOP1 Recombinant Human although stable at 4℃ for 30 days， should be stored desiccated below -20℃ for periods greater than 30 days. Please avoid freeze-thaw cycles.
其他
ProSpec 是一家全球著名細(xì)胞因子蛋白生產(chǎn)和研發(fā)的公司，具有20多年的生產(chǎn)經(jīng)驗(yàn)，專注于蛋白（重組及合成）生產(chǎn)研發(fā)。我們的核心產(chǎn)品包括：干擾素、白介素、骨蛋白、腫瘤壞死因子、成纖維生長因子、瘦素、干細(xì)胞因子、催乳素、表皮生長因子、胰島素樣生長因子、神經(jīng)生長因子、集落刺激因子、激素、酶、病毒抗原以及各種重組蛋白等。 生產(chǎn)工藝和規(guī)模使其可以提供毫克到克級(jí)蛋白，價(jià)格優(yōu)于同類公司。絕大部分產(chǎn)品是天然成熟型蛋白，而不含有標(biāo)簽蛋白。考慮到大部分研究者希望能更靈活地配制蛋白溶液，ProSpec-Tany絕大部分產(chǎn)品沒有添加保護(hù)劑或鹽，這就對(duì)工藝提出了更高的要求。同時(shí)，ProSpec-Tany絕大部分產(chǎn)品為凍干粉，因此易于運(yùn)輸和保存。
