艾美捷MethylFlash 5-mC RNA甲基化定量分析試劑盒（熒光檢測）是一套完整的優化緩沖液和試劑，用于熒光定量總5-甲基胞嘧啶（5-mC）RNA甲基化水平。 將 RNA結合至 strip-wells，使用 5-mC RNA 辨認抗體與偵測抗體然后進行熒光成色反應，利用熒光光譜進行熒光強度測定甲基化修飾換算。

艾美捷MethylFlash 5-mC RNA甲基化定量分析試劑盒 (Fluorometric)包含 5-mC RNA 甲基化定量實驗所需的所有最佳化試劑，可以使用任何物種的 total RNA 樣品來進行實驗，如哺乳動物、植物、真菌、細菌和病毒等，無論是從培養細胞、新鮮或冷凍的組織、血漿/血清或體液等來源皆可適用。  

艾美捷MethylFlash 5-mC RNA甲基化定量分析試劑盒特點：

快速 – 全部反應只需2小時40分

高效 – 穩定的品質讓您的數據擁有高度重復性

方便 – 流程簡單快速

高敏感性 – 可從200 ng的RNA樣品偵測到至低0.02%的5-mC RNA 修飾

專一性 – 高專一性抗體使用, 不會有其他unmethylated cytosine或 hydroxymethylated cytosine的交互辨識干擾

均一性 – 套組中皆附有positive和negative control

準確性 – 極-佳的positive control設計可讓您的實驗數據達到如HPLC-MS分析實驗的效能

彈性化 – 採用strip-well的microplate格式, 能依照實驗需求來進行少量或是高通量實驗

5-mC RNA甲基化定量分析試劑盒引文賞析：

Betlej G et. al. (October 2022). RNA 5-methylcytosine status is associated with DNMT2/TRDMT1 nuclear localization in osteosarcoma cell lines. J Bone Oncol. 36:100448.

Bataglia L et. al. (July 2021). Active genic machinery for epigenetic RNA modifications in bees. Insect Mol Biol.

Park MH et. al. (January 2020). Mono-(2-Ethylhexyl)phthalate Regulates Cholesterol Efflux via MicroRNAs Regulated m⁶A RNA Methylation. Chem Res Toxicol.

Lewinska A et. al. (August 2017). Reduced levels of methyltransferase DNMT2 sensitize human fibroblasts to oxidative stress and DNA damage that is accompanied by changes in proliferation-related miRNA expression. Redox Biol. 14:20-34.

Lewinska A et. al. (February 2017). Downregulation of Methyltransferase Dnmt2 Results in Condition-dependent Telomere Shortening and Senescence or Apoptosis in Mouse Fibroblasts. J Cell Physiol.